Olympus Multiphoton FV MPE RS Apollo System: With the Olympus FVMPE-RS, countless possibilities for deep tissue observation are finally realized. The system delivers unmatched high-speed imaging, essential for capturing the dynamic in vivo response, with fine laser control pinpointing the precise site for optimum excitation efficiency — even deep within the sample. Accompanied by high S/N-ratio imaging and dedicated Olympus multiphoton objectives, efficient detection of scattered fluorescence photons is also ensured. This upright microscope is coupled with a Spectre Physics Insight DS Dual (680-1300nm) laser. In essence, the Olympus FLUOVIEW FVMPE-RS unites high-speed, deep observation capability with multicolor imaging and powerful laser light stimulation, offering a no compromise solution to answer innovative biological research questions.
Zeiss LSM 710 Confocal: The LSM 710 is an inverted confocal microscope equipped to handle all your fixed tissue imaging needs. A confocal uses point illumination to help overcome some of the limitations of traditional wide-field fluorescence microscopes, including improved optical resolution at depth, decreased background fluorescence and thin optical sectionining for 3D reconstructions. It can collect transmitted light images (bright field and DIC), as well as conventional and confocal fluorescence images. The microscope is a top of the line spectral point scanner equipped with the following laser lines (405, 458, 488, 514, 561, and 633nm) software for image processing and analysis are also available for use.
Zeiss AXIO M2 Imager Epifluorescent: This is a fully upright motorized microscope including motorized stage that allows slide scanning (tiling) and acquisition of Z-stacks. The instrument is equipped with 4 filters that detect DAPI, GFP/488, Cy3 and Cy5 wavelengths. An Axiocam 105 digital color camera and a high resolution Axiocam 506 digital monochrome camera are installed on the microscope allowing acquisition of either color or monochrome images. Zen Image analysis software that includes deconvolution, colocalization and extended depth of focus is available on the instrument workstation and on a second workstation dedicated to image processing.