FACSAria II (BD Biosciences, San Jose, CA): 3 laser system capable of high throughput sorting (25000 EPS) with greater than 99% purity. It also has high performance analysis capabilities for assays like cell proliferation, immunophenotyping, apoptosis, etc. It can perform aseptic sorting with 4 different markers at one time on tubes, slides and micro well plates. It's capable of single cell or specific number of cells sorting. Improvements in fluidics and optics have allowed for higher speed sorting (25,000 events/sec at 70psi using a 70um nozzle) with enhanced overall sensitivity. The system uses fiber optic guided solid state 488nm, 635nm and 405nm diode lasers along with fiber guided emission collections (four colors plus scatter off the 488nm and two colors off the 635nm line and two colors off of the 405nm line). These traits allow for an instrument that does not require the extensive alignment optimizations inherent to the jet-in-air sorters making for increased reproducibility in identifying and sorting dim population.
Canto II (BD Biosciences, San Jose, CA): A 3 laser 8 Color Analyzer with 488nm, 647nm and 405nm wavelength laser system. It is capable of highperformance analysis for assays like cell proliferation, immunophenotyping, cell cycle, apoptosis etc. BD FACS Canto system is an easy-to-use benchtop analyzer that delivers proven performance, accuracy and high-quality results. The BD FACSCanto II features many innovations, including a true fixed alignment flow cell to minimize startup time and improve reproducibility. The optical system features a patented design that maximizes signal detection and increases sensitivity and resolution for each color in a multicolor assay.
Lyse Wash BD Biosciences, San Jose, CA): The BD FACS™ Lyse Wash Assistant (LWA), with its patented cell wash technology, automates sample preparation for flow cytometry applications. It lyses, mixes, washes, and fixes samples with minimal labor. Automating incubation and washing, the LWA batch processes up to 40 patient samples per run to reduce hands-on time and operatorinduced variability in laboratory data. Samples can be transferred to a BD FACSCanto or BD FACSCalibur flow cytometer for analysis using the LWA carousel.