What is Flow Cytometry?

Flow CytometryFlow Cytometry is a technology for measuring cells or particles as they move in a liquid stream through a laser or light beam past the point of interrogation. Cytometry measures the relative light scattering and color discrimination fluorescence of the particles.

The cells are analyzed and differentiated based on size and granularity and whether the cell is carrying fluorescent molecules, either in the form of antibodies or dyes.

The modern flow cytometer consists of a light source, collection optics, electronics and a computer to translate signals to data. The light source of choice is a laser, which emits coherent light at a specified wavelength. Scattered and emitted fluorescent light is collected by two lenses, one set in front of the light source (forward scatter) and one set at right angles (side scatter) and by a series of optics, beam splitters and filters, specific bands of fluorescence can be measured.

Flow cytometers uses several types of laser — like the gas lasers (e.g. helium-neon, argon, helium-cadmium), solid state lasers (e.g. NdYAG), dye lasers or semi-conductor lasers.

The choice of fluorochrome to be used is influenced both by the application and the excitation wavelengths and the availability of an appropriate laser. It is possible to measure physical characteristics such as cell size, shape and internal complexity and any cell component or function that can be detected by a fluorescent compound. The applications of flow cytometry are numerous, and this has led to widespread use of these instruments in the biological and medical fields.

Mission

To be the premier cell analysis resource for the community of Principal Investigators (PIs) at University of Arizona College of Medicine - Phoenix, and local research institutions. The goal is to advance health care and innovation through an exchange of information between PIs, students, and staff in the development of new, cutting-edge applications of flow cytometry technology.

Services

The Flow Cytometry Core provides expertise, training/education, personnel and instrumentation, to assist users in multi-parametric flow cytometry, and fluorescence-activated cell sorting (FACS) for both basic and clinical research. The core Director can provide help with multicolor/multiparameter panel designing, design of experiments, as well as to develop and implement new applications/assays. The core staff is available to provide assistance in sample preparation, staining, data acquisition and analysis.

The Flow Cytometry Core offers an array of services across the spectrum of translational research, from basic medical science to clinical trials. These services include experimental design, sample preparation, data analysis and consultation and many other types of collaborative work. 

Collaborations

Research Applications

  • Immunophenotyping.
  • Cell sorting.
  • Cell cycle analysis.
  • Phosphorylated proteins.
  • Cytoplasmic/nuclear antigen analysis.
  • Apoptosis.
  • Cell proliferation assays.
  • Intracellular calcium flux.
  • Viability.
  • Cytometric bead array CBA technology.
  • FloCytoMix multiple analyte detection dystem.
  • Neurons.
  • Exosomes.