Comprehensive interpretation of behavioral outcomes requires integrating complementary methodologies that probe neural activity, brain circuitry and molecular changes.
- In vivo neural activity monitoring captures real-time neuronal firing patterns during behavior, revealing how specific brain regions and circuits drive behavioral responses.
- Intracranial surgery, including stereotactic injection of viral vectors, enables precise manipulation of neural circuits through optogenetic or chemogenetic modulation, and neural recordings. Stereotactic delivery of viral vectors facilitates brain cell type-specific manipulation and circuit mapping to establish causal relationships between brain activity and behavior.
- Histology and Microscopy provide essential insights into neural architecture, cellular organization and molecular changes, offering anatomical context to behavioral outcomes. Histological analysis can be useful to validate viral vector expression, assess cannula placement and reveal structural or molecular changes within specific neural populations.
- Data and video analysis play a pivotal role in quantifying behavioral metrics, detecting patterns and deriving meaningful insights from complex datasets in real time and offline.
Together, these capabilities create a powerful framework for dissecting the neural mechanisms underlying behavior and advancing neuroscience research.
To learn more about the associated capabilities or to discuss specific experimental needs, please contact Dr. Ayanabha Chakraborti