Flow Cytometry Core - Instrumentation

Canto II

Canto II (BD Biosciences, San Jose, CA): A three laser, eight Color Analyzer with 488nm, 647nm and 405nm wavelength laser system. It is capable of high-performance analysis for assays like cell proliferation, immunophenotyping, cell cycle, apoptosis etc. BD FACS Canto system is an easy-to-use benchtop analyzer that delivers proven performance, accuracy and high-quality results. The BD FACSCanto II features many innovations, including a true fixed alignment flow cell to minimize startup time and improve reproducibility. The optical system features a patented design that maximizes signal detection and increases sensitivity and resolution for each color in a multicolor assay.

Lasers:

• Air-cooled:
  • 405-nm solid state diode, 30-mW fiber power output.
  • 488-nm solid state, 20-mW laser output.
  • 633-nm HeNe, 17-mW laser output.

Detectors and Dyes:

• Violet: 450/50 (Pacific Blue™ or DAPI or Hoechst or BV421 or V450); 502 to 525 nm (AmCyan, or BV510 or V500c).
• Blue: 530/30; 585/42; >670; 780/60 nm; FITC, PE, PerCP or PerCP-Cy5.5 PE-Cy7 (525, 575, 678 or 695, 785 nm).
• Red: 660/20 (APC); 780/60 nm (APC-Cy7/APC-H7).

Guava easyCyte 8 High Throughput System (HTS)

Guava easyCyte 8 High Throughput System (HTS): The easyCyte 8HT instrument pair the power of a 150 mW blue laser (488) with the expanded multi-channel capacity of a red laser (642) to provide six channels plus FSc and SSc, for a total of eight parameters. The guava easyCyte™ HT instruments provide high throughput analysis with a robotic sample tray that automatically handles a 96-well micro-plate and up to 10 sample tubes. It has a patented, micro-capillary flow cell that eliminates the need for sheath fluid and provides absolute cell counts. This translates into less complexity, smaller samples and minimal waste, saving you both time and money. Plus, the flow cell is self-aligning and user-replaceable to reduce downtime and service visits.

• No laser alignment or sheath fluid required.
• Uses smaller sample volume and generates less waste.
• Flow cell is user replaceable for minimal downtime.
• Aspirates sample directly from the tube.
• Absolute Cell Counting and Viability.

Lasers and Detectors:

• 488nm Laser- RED-R (661/15), NIR-R (785/70).
• 642 nm Laser- GRN-B (525/30), YEL-B (583/26), RED-B (695/50), NIR-B (785/70).

Detectors and Dyes:

• GRN-B: FITC or Alexa 488 or GFP or SYBERgreen or Sytox green or CFSE.
• YEL-B: Alexa 555 or PE or sytox orange or CFSE or sybergreen.
• RED-R: Alexaflour 647 or APC or CD647 or cy5 or Qdot655 or DRAQ5 or EtBr or sytoxred or TO-PRO-3.
• RED-B:7AAD or PI, PEAlexa647 or PE alexa700, Pecy5 or Pecy5.5, PercP or PercPcy5.5.
• NIR-B: PE-Alexaflour750 or PI or Pecy7.
• NIR-R: APC-Cy7 or APC-alexaflour750.

Lyse Wash

Lyse Wash BD Biosciences, San Jose, CA): The BD FACS™ Lyse Wash Assistant (LWA), with its patented cell wash technology, automates sample preparation for flow cytometry applications. It lyses, mixes, washes, and fixes samples with minimal labor. Automating incubation and washing, the LWA batch processes up to 40 patient samples per run to reduce hands-on time and operator-induced variability in laboratory data. Samples can be transferred to a BD FACSCanto or BD FACSCalibur flow cytometer for analysis using the LWA carousel.