The Wire Hanging Test is a behavioral assay to evaluate muscular strength, grip endurance, and neuromuscular function in rodent models. This test is based on the principle that a rodent's natural tendency is to grasp and hang onto an elevated wire or rod when suspended by its forelimbs. During the test, the animal is placed on a horizontal wire suspended above a soft padded surface. The wire is typically elevated 30-40 cm above the padding to discourage the animal from dropping off voluntarily. The test begins when the animal is allowed to grasp the wire with its forelimbs, and a stopwatch is started. Key parameters measured include the latency to fall, which represents the time the animal can maintain its grip on the wire before releasing and falling onto the padded surface below. Typical test durations range from 60 seconds to 5 minutes, with a maximum cut-off time enforced to prevent excessive fatigue or stress.